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second generation high throughput second generation sequencing  (Complete Genomics Inc)


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    Complete Genomics Inc second generation high throughput second generation sequencing
    Second Generation High Throughput Second Generation Sequencing, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 99/100, based on 2118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/second+generation+sequencing/DNBSEQ-T7/pmc12772332-211-11-16
    Average 99 stars, based on 2118 article reviews
    second generation high throughput second generation sequencing - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Integrated Analysis of Transcriptome and Metabolome Reveals the Accumulation of Anthocyanins in Black Soybean ( Glycine max L.) Seed Coats Induced by Low Nitrogen Concentration in the Nutrient Solution.
    Article Snippet: Using the fragmented mRNA as a template, double-stranded cDNA was synthesized via reverse transcriptase, followed by terminal repair with an End Repair Mix (ABclonal Biotechnology Co., Ltd., Woburn, MA, USA) and addition of an adenine (A) base to the 3′ end to form a “Y”-shaped adapter. .. Library enrichment was performed by PCR am- plification for 15 cycles (Fast RNA-seq Lib Prep Kit V2, Cat. No. RK20306) (ABclonal Biotechnology Co., Ltd., Woburn, MA, USA), after which sequencing was carried out using a second-generation high-throughput sequencing platform (DNBSEQ-T7, Shenzhen MGI Tech Co., Ltd., Shenzhen, China). ..

    Article Title: Integrated Analysis of Transcriptome and Metabolome Reveals the Accumulation of Anthocyanins in Black Soybean ( Glycine max L.) Seed Coats Induced by Low Nitrogen Concentration in the Nutrient Solution
    Article Snippet: Using the fragmented mRNA as a template, double-stranded cDNA was synthesized via reverse transcriptase, followed by terminal repair with an End Repair Mix (ABclonal Biotechnology Co., Ltd., Woburn, MA, USA) and addition of an adenine (A) base to the 3′ end to form a “Y”-shaped adapter. .. Library enrichment was performed by PCR amplification for 15 cycles (Fast RNA-seq Lib Prep Kit V2, Cat. No. RK20306) (ABclonal Biotechnology Co., Ltd., Woburn, MA, USA), after which sequencing was carried out using a second-generation high-throughput sequencing platform (DNBSEQ-T7, Shenzhen MGI Tech Co., Ltd., Shenzhen, China). ..

    RNA Sequencing:

    Article Title: Integrated Analysis of Transcriptome and Metabolome Reveals the Accumulation of Anthocyanins in Black Soybean ( Glycine max L.) Seed Coats Induced by Low Nitrogen Concentration in the Nutrient Solution.
    Article Snippet: Using the fragmented mRNA as a template, double-stranded cDNA was synthesized via reverse transcriptase, followed by terminal repair with an End Repair Mix (ABclonal Biotechnology Co., Ltd., Woburn, MA, USA) and addition of an adenine (A) base to the 3′ end to form a “Y”-shaped adapter. .. Library enrichment was performed by PCR am- plification for 15 cycles (Fast RNA-seq Lib Prep Kit V2, Cat. No. RK20306) (ABclonal Biotechnology Co., Ltd., Woburn, MA, USA), after which sequencing was carried out using a second-generation high-throughput sequencing platform (DNBSEQ-T7, Shenzhen MGI Tech Co., Ltd., Shenzhen, China). ..

    Article Title: Integrated Analysis of Transcriptome and Metabolome Reveals the Accumulation of Anthocyanins in Black Soybean ( Glycine max L.) Seed Coats Induced by Low Nitrogen Concentration in the Nutrient Solution
    Article Snippet: Using the fragmented mRNA as a template, double-stranded cDNA was synthesized via reverse transcriptase, followed by terminal repair with an End Repair Mix (ABclonal Biotechnology Co., Ltd., Woburn, MA, USA) and addition of an adenine (A) base to the 3′ end to form a “Y”-shaped adapter. .. Library enrichment was performed by PCR amplification for 15 cycles (Fast RNA-seq Lib Prep Kit V2, Cat. No. RK20306) (ABclonal Biotechnology Co., Ltd., Woburn, MA, USA), after which sequencing was carried out using a second-generation high-throughput sequencing platform (DNBSEQ-T7, Shenzhen MGI Tech Co., Ltd., Shenzhen, China). ..

    Sequencing:

    Article Title: Integrated Analysis of Transcriptome and Metabolome Reveals the Accumulation of Anthocyanins in Black Soybean ( Glycine max L.) Seed Coats Induced by Low Nitrogen Concentration in the Nutrient Solution.
    Article Snippet: Using the fragmented mRNA as a template, double-stranded cDNA was synthesized via reverse transcriptase, followed by terminal repair with an End Repair Mix (ABclonal Biotechnology Co., Ltd., Woburn, MA, USA) and addition of an adenine (A) base to the 3′ end to form a “Y”-shaped adapter. .. Library enrichment was performed by PCR am- plification for 15 cycles (Fast RNA-seq Lib Prep Kit V2, Cat. No. RK20306) (ABclonal Biotechnology Co., Ltd., Woburn, MA, USA), after which sequencing was carried out using a second-generation high-throughput sequencing platform (DNBSEQ-T7, Shenzhen MGI Tech Co., Ltd., Shenzhen, China). ..

    Article Title: The expansion of culturable opportunistic pathogens and antibiotic resistance in mouse gut following antibiotic exposure
    Article Snippet: .. Genomic DNA was extracted from the 80 strains and subject to second-generation high-throughput second generation sequencing (BGI DNBSEQ-T7 at PE150 mode). ..

    Article Title: Integrated Analysis of Transcriptome and Metabolome Reveals the Accumulation of Anthocyanins in Black Soybean ( Glycine max L.) Seed Coats Induced by Low Nitrogen Concentration in the Nutrient Solution
    Article Snippet: Using the fragmented mRNA as a template, double-stranded cDNA was synthesized via reverse transcriptase, followed by terminal repair with an End Repair Mix (ABclonal Biotechnology Co., Ltd., Woburn, MA, USA) and addition of an adenine (A) base to the 3′ end to form a “Y”-shaped adapter. .. Library enrichment was performed by PCR amplification for 15 cycles (Fast RNA-seq Lib Prep Kit V2, Cat. No. RK20306) (ABclonal Biotechnology Co., Ltd., Woburn, MA, USA), after which sequencing was carried out using a second-generation high-throughput sequencing platform (DNBSEQ-T7, Shenzhen MGI Tech Co., Ltd., Shenzhen, China). ..

    Article Title: An Asian case of combined 17α-hydroxylase/17,20-lyase deficiency due to homozygous p.R96Q mutation: A case report and review of the literature
    Article Snippet: .. Genomic DNA was extracted from peripheral blood leukocytes using a Blood DNA mini kit (Qiagen, Germany); DNA fragments in the target region were enriched using a customized capture kit (MyGenostics, Beijing, China) and sequenced using a high-throughput second-generation sequencing platform (DNBSEQ-T7, MGI, Shenzhen, China). ..

    Next-Generation Sequencing:

    Article Title: Integrated Analysis of Transcriptome and Metabolome Reveals the Accumulation of Anthocyanins in Black Soybean ( Glycine max L.) Seed Coats Induced by Low Nitrogen Concentration in the Nutrient Solution.
    Article Snippet: Using the fragmented mRNA as a template, double-stranded cDNA was synthesized via reverse transcriptase, followed by terminal repair with an End Repair Mix (ABclonal Biotechnology Co., Ltd., Woburn, MA, USA) and addition of an adenine (A) base to the 3′ end to form a “Y”-shaped adapter. .. Library enrichment was performed by PCR am- plification for 15 cycles (Fast RNA-seq Lib Prep Kit V2, Cat. No. RK20306) (ABclonal Biotechnology Co., Ltd., Woburn, MA, USA), after which sequencing was carried out using a second-generation high-throughput sequencing platform (DNBSEQ-T7, Shenzhen MGI Tech Co., Ltd., Shenzhen, China). ..

    Article Title: Integrated Analysis of Transcriptome and Metabolome Reveals the Accumulation of Anthocyanins in Black Soybean ( Glycine max L.) Seed Coats Induced by Low Nitrogen Concentration in the Nutrient Solution
    Article Snippet: Using the fragmented mRNA as a template, double-stranded cDNA was synthesized via reverse transcriptase, followed by terminal repair with an End Repair Mix (ABclonal Biotechnology Co., Ltd., Woburn, MA, USA) and addition of an adenine (A) base to the 3′ end to form a “Y”-shaped adapter. .. Library enrichment was performed by PCR amplification for 15 cycles (Fast RNA-seq Lib Prep Kit V2, Cat. No. RK20306) (ABclonal Biotechnology Co., Ltd., Woburn, MA, USA), after which sequencing was carried out using a second-generation high-throughput sequencing platform (DNBSEQ-T7, Shenzhen MGI Tech Co., Ltd., Shenzhen, China). ..

    Amplification:

    Article Title: Integrated Analysis of Transcriptome and Metabolome Reveals the Accumulation of Anthocyanins in Black Soybean ( Glycine max L.) Seed Coats Induced by Low Nitrogen Concentration in the Nutrient Solution
    Article Snippet: Using the fragmented mRNA as a template, double-stranded cDNA was synthesized via reverse transcriptase, followed by terminal repair with an End Repair Mix (ABclonal Biotechnology Co., Ltd., Woburn, MA, USA) and addition of an adenine (A) base to the 3′ end to form a “Y”-shaped adapter. .. Library enrichment was performed by PCR amplification for 15 cycles (Fast RNA-seq Lib Prep Kit V2, Cat. No. RK20306) (ABclonal Biotechnology Co., Ltd., Woburn, MA, USA), after which sequencing was carried out using a second-generation high-throughput sequencing platform (DNBSEQ-T7, Shenzhen MGI Tech Co., Ltd., Shenzhen, China). ..



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